Sex identification in birds based on polymerase chain reaction (PCR) and polyacrylamide gel electropheresis (PAGE). - Mujtaba G., Nadeem M.S. - Berkut. 15 (1-2). 2006. - P. 194-196.
PCR and PAGE techniques could be used to identify sexes in birds, where females are heterogametic (ZW) and males are homogametic (ZZ). The sex system depends upon a female specific W-chromosome linked gene (CHD-W) and a male specific Z-chromosome linked gene (CHD-Z). The two genes could be amplified by PCR, and give different banding pattern on Polyacrylamide gel in a wide variety of birds. We examined 180 birds of six different species, identified their sex using this approach. This technique could be universally used to identify sexes of most avian species. [English].
Key words: biochemistry, chromosome, gene, PCR, PAGE, sex.
Address: G. Mujtaba, Biochemistry Department, Hazara University, Mansehra, Pakistan;